The brilliance of discovery
High Sensitivity Fluorescence
In 2000 the Nobel Prize in Chemistry was awarded for the discovery of a unique class of conducting polymer materials which are revolutionizing the electronic and display industries and are the foundation of Sirigen’s™ technology.
Fluorescent detection is the method of choice for numerous Diagnostic and Research applications. Reagents are stable, the method is familiar and operators are used to performing fluorescent assays and there is a large existing base of suitable installed hardware in routine use. Fluorescence offers potentially the simplest route to multiplex analysis.
However, for numerous applications conventional fluorescent reagents limit assay sensitivity or impose the requirement for complex and expensive instrumentation to deliver the required performance. Generally the performance of fluorescent detection is limited by the performance of the reporter dyes used, most especially their ability to absorb excitation light. HSF™ Polymers address this shortfall specifically and are designed for maximal absorption of exciting light and conversion to fluorescent signal.
Typically HSF™ technology increases assay signal 1-2 orders of magnitude without increasing background. This gives a meaningful and transformative improvement in signal to noise ratio providing lower limits of detection, improved reproducibility and reduced assay “grayzones”.
HSF™ polymers are available in a variety of colors and formats all of which deliver the advantages of polymeric reporters making them the reagents of choice for multiplexing.
Sirigen has been able to demonstrate these benefits in a wide range of applications.